Development of a single drug, whether it is a new chemical entity or a biologic therapeutic, requires significant investment of resources. Each step of the process from early discovery through production and delivery must be fully explored, characterized, and understood.
From target discovery and validation to formulation and manufacturing, Enzo offers a line of products and services that can accelerate your drug discovery program. Choose from an extensive range of assay kits, enzymes, substrates and compound libraries for primary and secondary screening, immunodetection assays for reliable biomarker detection, and cytotoxicity assays for in vitro drug safety assessment. Our aim is to deliver innovative tools that make drug development more efficient, more cost-effective and more successful.
Streamline Your Screening Process With Over 3,000 Unique Small Molecules
Enzo Life Sciences has a long and successful track record in identifying, synthesizing, and commercializing valuable known bioactives for use as research tools. Our small molecules are available as either stand-alone products or curated libraries, offering ultimate flexibility and value. Our long-standing, flagship SCREEN-WELL? Compound Library product family offers an easy, ready-to-use method for compound screening.
Accelerate drug optimization with a unique collection of compounds of known safety and bioavailability for diverse targets
Compounds can be used for diverse drug discovery in the fields of cardiology, neuropsychiatry, immunology, oncology and more!
Contains more than 100 additional compounds compared to our previous FDA approved library versions
100% known bioactivity and greatest degree of druglikeness available
Recently approved FDA compounds are also included
We provide detailed information in our comprehensive documentation for each compound
Visualize Cellular Responses With Novel Fluorescent Probes
Our panel of CELLESTIAL? fluorescence-based live cell assays is built upon our extensive expertise in the design and synthesis of fluorescent probes. From simple organelle specific dyes for imaging cell structure and determining cell viability, to more complex dyes for evaluating cell signaling and death pathways, each of our probes is developed to provide sensitivity, specificity, and convenience.
Specific – Reduce false positives by eliminating non-specific dye-associated artifacts
Flexible – Compatible with common dyes and fluorescent markers (i.e., GFP) for multiplex analysis
Consistent – Optimized for reproducibility on microplate, flow cytometry, or fluorescent microscopy platforms
Screen Enzyme Modulators With Innovative Activity Assays
Our decades of experience in the design and manufacture of active enzymes and their substrates supports development of an ever-expanding portfolio of biochemical assays. Our menu of scalable enzyme activity assays is anchored by our Fluor de Lys? deacetylase assay platform, featuring kits for screening modulators of HDAC and Sirtuin activity. We also offer FRET-based assays for matrix metalloproteinases (MMPs) and caspase activity, as well as phospho-specific antibody-based kinase assays. These assays exemplify the synergy of Enzo capabilities, assembling small peptide, active recombinant protein synthesis, fluorescent labeling and detection technologies, and antibody development into robust, reproducible assay kits.
High activity, high purity recombinant enzymes for high-quality hits
Bulk enzymes and substrates available for post-screening studies
Patented substrate/developer chemistries in a variety of readout formats
Green & chemiluminescent assays available to minimize interference
Assay all HDAC classes from multiple sources
High-activity, in-house manufactured control enzymes
Convenient Screening Formats
MMP RED and GREEN Drug Discovery Kits
Improved red-shifted substrate with better kinetics and brighter signal
Includes active recombinant enzyme, substrate, and assay buffer
Convenient real-time kinetics of cleavage is easily determined
Microplate format for high-throughput screening
Fluorescent emission spectra of small molecule drug camptothecin (light blue), OE33 cells (dark blue), MMP GREEN substrate (green), and MMP RED substrate (red), showing that the MMP GREEN and RED substrates avoid the interference caused by small molecules and cells.
Akt, PKA, PKC Kinase Activity Kits
Optimal flexibility with end-point or kinetic assay read-out
Safe, rapid and reliable colorimetric detection for use with basic instrumentation
Validated for multiple sample types including adherent cells, suspension cells, tissue extracts, and proteins from any species
Maximal efficiency and low sample volume requirements leading to reduced cost during screening
Analysis of purified active PKA activity with ADI-EKS-390A. Varying quantities of purified active PKA were assayed using the PKA substrate microtiter plate. Assays were incubated for 60 min at 30° C.
Detect Analytes Accurately With High Sensitivity ELISAs
Enzo Life Sciences offers a large variety of immunoassay kits in both immunometric and competitive assay formats. Each kit is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specificity. You can be confident that you will obtain reproducible results, day-after-day and lot-after-lot.
Consistent – strict quality control guidelines ensure reproducible results, lot-after-lot
Trusted – reliability highlighted in peer-reviewed publications for three decades
Flexible – adaptable to automated equipment for high throughput systems